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Ultraviolet Visible spectroscopy (UV-Vis spectroscopy) is an analytical technique, which measures the amount of discrete wavelengths of ultraviolet-visible light absorbed or transmitted through a sample in comparison to a blank or reference. It relies on the use of light. Absorbance and transmittance properties are influenced by composition of sample, providing information on what is in the sample and in what concentration.
Some strengths of UV-Vis spectroscopy make it so popular for many applications. They are as follows:
Having introduced you to the technique and its advantages, this article elaborates on different applications of UV-Vis spectroscopy in different fields.
The applications of UV-Vis spectroscopy are enormous. The following are the main fields in which UV-Vis spectroscopy is used:
Uv-Vis spectroscopy deals with the purity of nucleic acids.
UV-Vis spectroscopy is an indispensable equipment in production of pharmaceutics.
UV-Vis spectroscopy is essential in the biomass growth curve studies.
Uv-Vis spectroscopy is also used in FMCG industries.
Besides, there are extensive applications of UV-Vis spectroscopy in other fields also. Below are the other applications of UV-Vis spectroscopy:
In addition to the above applications, there are still some fields where UV-Vis spectroscopy is widely used.
The applications of UV-Vis spectroscopy are seemingly endless. Still, there are few limitations for UV-Vis spectroscopy. Presence of stray light may cause measurement errors. Presence of suspended solids and bubbles in the cuvette can cause scattering. Also, geometrical considerations are significant to obtain reproducible results. Since, there will be interference from multiple absorbing species, each chemical species should be isolated from the sample measured individually for proper quantitative analysis.