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Absorbance studies have become a base for the examination of numerous particles and solutions. For this to happen, light needs to be passed in a defined format. The format is provided by cuvette. The selection of the cuvette is a significant element in the measurement of absorbance in the UV-Vis spectrophotometer as solutions are placed inside the cuvette for analysis. Also, the selection is a delicate task as there are varieties of cuvettes available. This blog gives an introduction to the cuvette. Then, types of cuvette are provided. It also speaks about the way of the usage of the cuvette. Subsequently, it discusses how different factors affect the selection of cuvette in the UV-Vis spectroscopy system.
Cuvettes are called by various different names. They are as follows:
. The popular cuvette materials are as follows:
When looking for a measurement of absorbance in a UV-Vis spectrophotometer, selecting a proper cuvette is an essential thing as each cuvette material has its own pros and cons. Depending on the application of UV spectroscopy, the material is chosen.
There are some precautions that need to be taken while using cuvette for measurement of absorbance.
Cuvette is able to influence the result of absorbance directly or indirectly. Choosing an appropriate cuvette for the measurement of absorbance and transmittance in UV-Vis spectroscopy requires utmost care. The quality of the cuvette should not be compromised, otherwise experimental results will be impacted. Additionally, care should be taken of obtaining good cuvettes within a stipulated budget.