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UV-Vis spectroscopy is a quantitative laboratory technique used for the measurement of the absorbance of light by a chemical compound. It is performed through measurement of the intensity of light, which passes through a sample with respect to the intensity of light through a reference. It is used particularly in ultraviolet and visible regions. The obtained light intensity versus wavelength is called the spectrum.
Sometimes, the spectrum obtained from the UV-Vis spectrophotometer need not be the same every time. The result may be erroneous. Reproducibility is missing if any parameters like temperature, pH, concentration are changed. Hence, it is essential to study the factors influencing UV-Vis spectroscopy, so that a clear picture will be obtained for careful measurement of the spectrum. This blog takes you to the study of variables, which affect the spectrum obtained from UV-Vis spectroscopy.
For measurement of absorbance and studies, there are numerous factors, which impact the results in ultraviolet visible spectroscopy. The factors are as follows:
The change in temperature of the sample plays a role in the spectrum.
The concentration of sample present is directly proportional to the intensity of light absorption, thus influencing the spectrum.
The change of pH of the solution and the aquatic environment has an influence on the spectrum obtained from UV-Vis spectroscopy.
To an extent, the choice of solvent also affects the spectrum.
The configuration of molecules also has a say in the spectrum.
Molecular conjugation plays a role in determination of spectrum.
There are so many factors that impact the absorbance studies in UV-Visible spectroscopy. Choosing a proper solvent and buffer will make the reading accurate. At the same time, optimal temperature and concentration also provide the absorbance value with precision. Similarly, solvent pH should be optimized before taking the reading.